APPLIED INSTRUMENTAL ANALYSIS II CTEC 2431

COURSE DESCRIPTION (catalog description)

Applied Instrumental Analysis II (2-8-4) Study of advanced topics in instrumental analysis. Topics include atomic absorption, inductively coupled plasma, nuclear magnetic resonance, gas chromatography/mass spectrometry, liquid chromatography, and infrared spectroscopy.

REQUIRED TEXT / MANUALS

Spectrometric Identification of Organic compounds, Silverstein and Bassler, John Wiley, 5th edition

The Spectra Workbook, Volume 4 by A.R. Gennaro and F.L. Bass-Bio-Rad Sadler Division, Sadler Research Laboratories Inc.

COURSE DESCRIPTION

Study of advanced topics in instrumentation analysis.  Topics include atomic absorption, inductively coupled plasma, nuclear magnetic resonance, gas chromatography/mass spectrometry, liquid chromatography, and infrared spectroscopy..

LEARNING OUTCOMES

The student will prepare and analyze samples; calibrate, operate, and maintain instruments; interpret data; describe operating principles of chemical instrumentation; and explain the operating principles and theory of chemical instrumentation.

COURSE OBJECTIVES

This course is designed to give the student an understanding in the operation and care of instruments used in chemical laboratories.

• To give the student an understanding of basic theory involved in the principles of chemical instrumentation.
• To teach the student to operate chemical instruments.
• To teach the student t set up and calibrate instruments used in the chemical laboratory.
• To emphasize the safe use of chemical instrumentation.
• To teach the student to solve spectral problems as they related to gas chromatography, mass spectrometry, infrared spectrometry, nuclear magnetic resonance spectrometry and ultraviolet spectrometry.

Syllabus

COURSE OUTLINE

LECTURE

ELECTRICAL COMPONENTS and CIRCUITS    

 

Lecture Notes

Powerpoint Presentations

• Laws of Electricity
  • Ohm's Law
  • Kirchoff's Law
  • Power Law
• Basic Direct Current Circuits
  • series
  • parallel
• Current, Voltage, and Resistance Measurements

DOUBLE BEAM SPECTROPHOTOMTERS

ATOMIC ABSORPTION 

• Introduction
• Ground and Excited State Atoms
• Theory of Atomic Absorption
• Principles of Operation
• Source Lamps
• Applications

ATOMIC ABSORPTION COMPONENTS

Lecture Notes

PowerPoint Presentations

• Source Lamps
• Nebulizers and Atomizers
• Monochromator Systems
• Detectors
• Single Beam and Double Beam
• Spectral Interferences

ATOMIC ABSORPTION APPLICATIONS

Lecture Notes

PowerPoint Presentations

• Sample Preparation
• Standard Curves and Calculations

INDUCED COUPLED PLASMA (ICP)

DETERMINATION OF MOLECULAR FORMULA

• Synthesis of Spectral Data

PROTON MAGNETIC RESONANCE SPECTROMETRY

• Introduction and Theory
• Apparatus and Sampling
• The Chemical Shift
• The NMR Spectrum
• Spin Spin Coupling
• The Anisotropic Effect
• Protons on Heteroatoms
• The Karplus Equation
• Long Range Spin Spin Splitting

INFRARED SPECTROMETRY

• Introduction and Theory
• Instrumentation
• Sampling Handling and Preparation
• IR Spectra of Paraffins (Normal, ISO and cyclic)
• Olefins and Acetylenes
• Aromatic Compounds
• Alcohols and Phenols
• Aldehydes, Ketones, Esters and Acids
• Ethers and Epoxides
• Acid Halides and Anhydrides
• Amines, Amides and other Nitrogen Compounds
• Organic Sulfur Compounds

MASS SPECTROMETRY

• Introduction and Instrumentation
• Fragmentation
• Mass Spectra of Some Chemical Classes
  • Alkanes
  • Iso-paraffins
  • Cycloparaffins
  • Alkenes (olefins)
  • McLafferty Rearrangement
• More Chemical Classes
  • Aromatic Compounds
  • The Tropylium Ion
  • Allylic, Vinylic and Benzylic Cleavage
• More Chemical Classes
  • Alcohols, Ethers and Amines
• More Chemical Classes
  • Aldehydes, Ketones, Esters and Acids
• Details of the McLafferty Rearrangement
• More Chemical Classes
  • Halogen Compounds
• Chemical Classes Concluded
  • Nitriles
  • Nitro compounds
  • Mercaptans

¹³C-NMR SPECTROMETRY

• Introduction and Spectra
  • Isotopes of Carbon
  • Th ¹³C-NMR spectrum
  • Decoupling and Spin-Spin Splitting
• More about ¹³C Spectra
• Solving problems

LABORATORY

USE OF THE PROTON N.M.R. SPECTROMETER (computer simulated)

• Instrument Adjusting
• Sample Handling
• Determining NMR Spectra
• Interpreting NMR Spectra

USE OF THE INFRARED SPECTROMETER

• Instrument Adjusting
• Sample Handling
• Determination of Spectra
• Interpreting of Spectra

OPERATION OF A GAS CHROMATOGRAPHIC MASS SPECTROMETER SYSTEM (namely, the Ion Trap).

SOLVING PROBLEMS INVOLVING ALL TYPES OF SPECTRA STUDIED IN THIS COURSE.

GRADING SYSTEM 

Lecture Grade =  Average of the Total Tests  +  Quiz Avg 

Lab Grade = Average of Labs 

Final Grade = Average of Lecture and Lab Grade 

The quiz average will be calculated with the two lowest quiz grade not included in the average.  Quizzes may not be made up if missed.  The two dropped quiz grades are used to compensate for this.  Major tests not taken on the announced test date will be recorded as a zero for grade computation purposes.  Major tests may not be made up if missed. 

A minimum grade of D must be made in both the lecture and laboratory component. If either component is below a D, a final grade of F will be assigned regardless of the final grade. 

The final letter grade is based on the final grade: 

90 - 100       =    A

80 -  89        =    B

70 -  79        =    C

60 -  69         =   D

below 60   =      F 

ATTENDANCE POLICY 

Refer to current Chemical Technology attendance policy. 

The student must be present for all tests, quizzes, and assignments.  Failure to attend will result in a grade of zero for that particular test, quiz or assignment.