APPLIED INSTRUMENTAL ANALYSIS I CTEC 1441

COURSE DESCRIPTION (catalog description)

Applied Instrumental AnalysisI (2-8-4) Overview of instrumental chemical analysis. Topics include chromatography, spectroscopy, and/or electroanalytical chemistry.

REQUIRED TEXT / MANUALS

Basic Gas Chromatography, H.M. McNair & E.J. Bonelli, Alltech Associates

Fundamentals of Analytical Chemistry, Skoog-West-Holler, Saunders Latest

COURSE OBJECTIVES

• This course is designed to give the student an understanding in the operation and care of instruments used in the chemical laboratories of industry.
• To introduce the student to principles and theory of instrument analysis.
• To teach the student the correct operation of chemical instruments.
• To introduce the student to the techniques of troubleshooting instruments in the chemical laboratory.
• To emphasize the safe use of chemical instrumentation.
• To teach the student to solve problems related to the use of chemical instruments.
• To stress proper record keeping in the chemical laboratory and plant.
• To encourage library use as applied to instrumental analysis.
• To perfect sampling techniques.
• To set the proper parameters (such as flowrate, current, temperature, etc.) on an industrial laboratory chromatograph.
• To properly install chromatographic columns.
• To use chromatography to make difficult separations of chemical mixtures.
• To perform quantitative analysis using a chromatogram.
• To integrate chromatographic peaks by several different methods.
• To make simple repairs and trouble shoot chromatographs.
• To make both practical and theoretical calculations.

COURSE OUTLINE (content)

LECTURE

ELECTRICAL COMPONENTS and CIRCUITS   

 

Lecture Notes

Lecture Videos and Powerpoint Presentations

• Laws of Electricity
  • Ohm's Law
  • Kirchoff's Law
  • Power Law
• Basic Direct Current Circuits
  • series
  • parallel
• Current, Voltage, and Resistance Measurements

QUANTITATIVE ANALYSIS

• Sources of error
• Integration
• Statistical treatment of data
• Sampling
• Storage

BRIEF HISTORY 

OVERVIEW OF CHROMATOGRAPHY 

• Basic Concepts
• Basic Terms
• Types of Chromatography
• Configurations

THE CHROMATOGRAM 

• Retention
• Peak Area
• Efficiency
• The Van Deemter Equation
• Capacity Factor
• Separation Factor
• Resolution
• Optimization

INSTRUMENTATION FOR GLC 

• Carrier gas
• Sample injection
• Oven
• Columns
• Stationary phases
• Detectors
  • TCD
  • FID
  • ECD

QUALITATIVE ANALYSIS

• Retention data
• Log plotting
• Retention index, I
• Relative detector response

RADIANT ENERGY AND MATTER   

 

Lecture Notes

Lecture Videos and Powerpoint Presentations

• Wave Properties
  • frequency
  • wavelength
  • wave number
  • amplitude
  • velocity
  • energy
• Interaction of Radiant Energy and Matter
  • ground energy states
  • excited energy states
• Dual Properties of Radiant Energy
• The Electromagnetic Spectrum
• Absorption Laws of Light
  • transmittance
  • absorption
  • Lambert-Bouguer Law
  • Beer's Law
  • absorptivity
  • deviations from Beer's Law
• Quantitative Use of Beer's Law

ULTRAVIOLET AND VISIBLE SPECTROPHOTOMETRY   

 

Lecture Notes

Lecture Videos and Powerpoint Presentations

SPECTROPHOTOMETER COMPONENTS    

 

Lecture Notes

Lecture Videos and Powerpoint Presentations

• Radiation Sources
• Monochromators
• Cells and Solvents
• Detectors
• Readout Devices

SPECTROPHOTOMETER INSTRUMENTS   

 

Lecture Notes

Lecture Videos and Powerpoint Presentations

• Filter Photometers
  • Single Beam
  • Double Beam
• Spectrophotometers
  • Single Beam
    • Optical Path
    • Electronics
    • Steps for Use
    • Advantages and Disadvantages
  • Double Beam
    • Optical Path
    • Electronics
    • Steps for Use
    • Advantages and Disadvantages
• Other Selections on Instruments

LABORATORY

ELECTRICAL

• Taking voltage, resistance, and current readings using a multimeter.
• Measuring voltage, resistance, and current in series circuits.
• Measuring voltage, resistance, and current in parallel circuits.

CHROMATOGRAPHY

• Set instrument parameters and develop injection technique
• Prepare a packed gas liquid column-pan coating method
• Determination of theoretical plate height (HETP) set flow rates
• Qualitative analysis
• Quantitative analysis
• Programmed temperature analysis
• Retention times for member of homoglous series
• Introduction to statistics and injection techniques
• Determination of water in ethanol by internal standardization
• Trouble shooting
• Thin layer chromatography
• Ion-exchange chromatography
• Paper chromatography

SPECTROPHOTOMETRY   

• Using a Spec 20 and its components.
• Measuring absorbance and transmittance.
• Beer’s Law (Cr determination)
• Cr/Co mixture.
• Iron in vitamins.
• Components and operation of a scanning uv/vis spectrophotometer.
• Individual student instruction on operation of uv/vis spectrophotometer
• Cr analysis using a scanning spectrophotometer.